murine ykl 40  (Boster Bio)

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Experimental feeding regimen.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Mouse Assay

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Hepatic insulin signal sensitivity is enhanced in Chi3L1 knockout mice fed with chow (CD). Western blot expression of ( A ) pAKT, ( B ) pGSK-3β, and ( C ) pERK proteins is enhanced in Chi3L1 knockout mouse liver 15 min after insulin injection (1 U/g body weight). WT: Wild type; CD: Chow diet; KO: knockout; pAKT: phosphorylated protein kinase B; AKT: Protein kinase B; pGSK-3β: phosphorylated glycogen synthase kinase 3 beta; GSK: glycogen synthase kinase; pERK: phosphorylated extracellular signal-regulated kinases; ERK: extracellular signal-regulated kinases.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Knock-Out, Western Blot, Expressing, Injection

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Lipid accumulation was reduced in Chi3L1 KO mice fed with HFD. Upper panel: representative Hematoxylin and eosin (H&E) staining, and demonstrate lipid accumulation in hepatocytes; Lower panel: representative Oil red O staining (ORO) and neutral fat accumulation in the murine liver (Bar = 20μm).

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Staining

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: High fat diet (HFD) increased Chi3L1 gene and protein expression. ( A ) Relative Chi3L1 messenger RNA expression measured by Real time PCR. Data are expressed as Mean ± Standard Error of Mean (SEM), p < 0.01 vs. CD fed mice, N = 8. ( B ) Chi3L1 protein levels in mice livers measured by Chi3L1 ELISA. Results in each group are expressed as Mean ± Standard Error of Mean (SEM), p < 0.001 vs. CD fed mice, N = 8.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Expressing, RNA Expression, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Effects of monoclonal anti-Chi3L1 antibody- FRG treatment on hepatic insulin signal transduction. ( A ) Hepatic insulin signaling in the murine liver measured by Western blot. High fat diet (HFD) fed mice were divided into two groups, five mice in each group were treated with either unrelated IgG or FRG (200 μg i.p twice/week) for 16 weeks. Three mice in each group were stimulated by insulin injection (1U/gram body weight) while the remainder were not injected with insulin before sacrifice. ( B , C ) Quantification by densitometry of pAKT and pERK expression in the mouse liver by Western blot analysis. p < 0.01, p < 0.001 vs. high fat diet + IgG treated mice, N = 3.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Transduction, Western Blot, Injection, Expressing

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Effect of anti-Chi3L1 mAbs on hepatic insulin signaling. ( A ) The expression of pAKT and AKT in the murine liver. The lower right panel showed the quantification by densitometry of Western blot results. ** p < 0.01, *** p < 0.001 vs. insulin (−) in the same group; ### p < 0.001 vs. CD insulin (+); & p < 0.05, && p < 0.01 vs. HFD insulin (+) ᶲᶲ p < 0.01, ᶲᶲᶲ p < 0.001 vs. HFD + IgG insulin (+), N = 4. ( B ) The expression of pGSK-3β and GSK in the murine liver. In the upper panel, the Western blot results for pGSK-3β and GSK protein expression are shown. The lower panel shows the quantification of Western blot data. * p < 0.05, ** p < 0.01 vs. insulin (−) in the same group; ### p < 0.001 vs. CD insulin (+); & p < 0.05, && p < 0.01, &&& p < 0.001 vs. HFD insulin (+), N = 4.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Expressing, Western Blot

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Monoclonal anti-Chi3L1 (FRG) antibody treatment reduced lipid accumulation in the mouse liver. ( A ) Representative H&E staining; ( B ) Representative Oil Red O (ORO) staining; ( C ) Quantitative analysis of ORO staining. All mice were treated with HFD for 16 weeks. The original magnification is 1000 x. p < 0.01 vs. HFD + IgG, N = 5. ( D ) Triglyceride content in the mouse liver tissue. Results were expressed as Mean ± Standard Error of Mean (SEM), p < 0.05 vs. HFD + IgG, N = 5 (Bar = 20 μm).

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Staining

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Monoclonal anti-Chi3L1 antibodies treatment reduced lipid accumulation in HFD fed WT C57BL/6 liver. ( A – E ) Representative H&E. Original magnification is 1000 x (Bar = 20 μm). ( F – J ) Representative Oil red O (ORO) staining (Bar = 20 μm). ( K ) Analysis of ORO staining for neutral fat accumulation in each group. The x-axis represents group information for each experimental setting, while the y-axis represents the percentile of the ORO area in the murine liver. *** p < 0.0001 vs. CD fed mice, # p < 0.05 vs. HFD+ unrelated IgG treated mice, N = 8. ( L ) Triglyceride content in the mouse liver. Results (horizontal bars) in each group were expressed as Mean ± Standard Error of Mean (SEM). Each symbol in the figure represents one animal in each group, N = 6.

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Staining

Journal: Cells

Article Title: Role of Chitinase 3-Like 1 Protein in the Pathogenesis of Hepatic Insulin Resistance in Nonalcoholic Fatty Liver Disease

doi: 10.3390/cells10020201

Figure Lengend Snippet: Representative Chi3L1 IHC staining for expression in human liver biopsy specimens. ( A ) NAFLD liver tissue. ( B ) NASH liver disease. Arrows indicated specific anti-Chi3L1 antibody stained cells including hepatocytes. Original magnification is 400× (Bar = 20 μm).

Article Snippet: Hepatic levels of Chi3L1 protein were measured with a murine Chi3L1/YKL-40 ELISA kit (Cat# LS-F2800-1, Lifespan Biosciences, Inc., Seattle, WA, USA).

Techniques: Immunohistochemistry, Expressing, Staining